{"id":"204fbdf9-3a37-44ea-9cca-0936eb3ed928","title":"A new understanding of somatic coliphages belonging to the Microviridae  family in urban wastewater","year":2025,"publisher":"ACTALIA","excerpt":"Somatic coliphages (SC) and F-specific RNA coliphages (FRNAPH) have been included in regulations or guide\u0002lines by several developed countries as a way of monitoring water safety and the microbiological quality of \nshellfish harvesting waters. SC are highly diverse in their morphology, size and geno…","url":"https://rd-agri.fr/detail/DOCUMENT/204fbdf9-3a37-44ea-9cca-0936eb3ed928","links":{"self":"/rd-agri/documents/204fbdf9-3a37-44ea-9cca-0936eb3ed928"},"description":"Somatic coliphages (SC) and F-specific RNA coliphages (FRNAPH) have been included in regulations or guide\u0002lines by several developed countries as a way of monitoring water safety and the microbiological quality of \nshellfish harvesting waters. SC are highly diverse in their morphology, size and genome. The Microviridae family \ncontains three genera of phages (Alphatrevirus, Gequatrovirus, and Sinsheimervirus), all having a capsid of similar \nmorphology (icosahedral) and size (25–30 nm in diameter) to that of common pathogenic enteric viruses. Three \nPCR assays specific for each genus of Microviridae were designed to study these phages in raw and treated \nwastewater (WW) in order to gain knowledge about the diversity and prevalence of Microviridae among SC, as \nwell as their inactivation and removal during WW treatments. Among the four wastewater treatment plants \n(WWTPs) monitored here, two WWTPs applied disinfection by UV light as tertiary treatment. First, we noticed \nthat Microviridae represented 10 to 30 % of infectious SC in both raw and treated WW. Microviridae appeared to \nbehave in the same way as all SC during these WW treatments. As expected, the highest inactivation, at least 4 \nlog10, was achieved for infectious Microviridae and SC in both WWTPs using UV disinfection. PCR assays showed \nthat the highest removal of Microviridae reached about 4 log10, but the phage removal can vary greatly between \nWWTPs using similar treatments. This work forms the basis for a broader evaluation of Microviridae as a viral \nindicator of water treatment efficiency and WW reuse.","language":"eng","keywords":[],"project":{"code":"19ART374748","name":"Mise au point d’une méthode d’estimation du caractère infectieux des norovirus humains dans les salades destinées aux marchés de la 1ère et 4ème gammes"},"notice-url":null,"document-url":"https://rd-agri.fr/rest/content/getFile/1ba26121-cdcc-4fff-80d0-290132d51bfe/Bichet+et+al-2024_A+new+understanding+of+coliphages+belonging+to+the+Microviridae+famili+in+urban+wastewater.pdf","productions":[],"taxa":[],"production-systems":[],"areas":[],"pests":[]}